A Versatile flowcell for automation of cyclic microscopy
A flowcell-based instrument to automate cyclic RNA/protein imaging of standard histology material without the need to manually manipulate the samples.
Technology
This tool allows for:
- The delivery of full automation of the iterative histology and imaging cycles, drastically reducing manual input cf. existing instruments (Bond Rx).
- Keep tissue sections in their respective flow cells throughout the entire experiment, minimizing tissue distortion due to repeated coverslip removal.
- Save reagent costs, target 20% (£10-12) per slide, across all cycles.
- Accept standard tissue slides rather than bespoke or custom-size tissue mounts (e.g. Akoya)
Advantages
- Minimises distortion: Keeps your sample intact between imaging runs.
- Compatible: The flowcell can be assembled on standard microscope slides and is compatible with most imaging systems. It can be used to process archive tissue.
- Cost-effective: This method would cut by four the time needed to process cyclic RNA/protein imaging of standard histology material.
- In parallel processing of ISH samples. Independent temperature control and liquid handling for maximum flexibility.
Applications/Context
- Automated Spatial Genomics
Comparable Technologies
All competitor products have been designed to address IHC as a primary assay, ISH is secondary and cyclic histology requires a great deal of manual input. Higher-plex ISH methods are still relatively new, and current equipment manufacturers do not adequately address user needs in this area.
Intellectual property
Priority patent application filed.
The Wellcome Sanger Institute is offering non-exclusive licenses to its IPR.
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